Can I read barcodes from existing files?

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Yes, the JavaScript SDK supports reading from a file in local memory. This can be achieved via the BarcodeReader class instead of the BarcodeScanner class.

let reader = await Dynamsoft.DBR.BarcodeReader.createInstance();
let results = await reader.decode(imageSource);

this article shows how to read barcodes from existing images and a list of supported input types.